Coupling between protonation and conformation in cytochrome c oxidase: Insights from constant-pH MD simulations
نویسندگان
چکیده
منابع مشابه
Insights into functions of the H channel of cytochrome c oxidase from atomistic molecular dynamics simulations.
Proton pumping A-type cytochrome c oxidase (CcO) terminates the respiratory chains of mitochondria and many bacteria. Three possible proton transfer pathways (D, K, and H channels) have been identified based on structural, functional, and mutational data. Whereas the D channel provides the route for all pumped protons in bacterial A-type CcOs, studies of bovine mitochondrial CcO have led to sug...
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C ytochrome c oxidase is the terminal oxidase in cellular respiration. This membrane protein accepts electrons from ferrocytochrome c in the periplasmic space of the mitochondrion, one electron at a time, and transfers the reducing equivalents to the binuclear heme-iron copper site (the socalled Fea3, CuB site), where dioxygen binds and the O·O bond is subsequently cleaved (1). In this manner, ...
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Cytochrome c oxidase vesicles were used to show that, under appropriate experimental conditions: (1) no net deprotonation of the vesicular membrane or of the incorporated enzyme occurs during the oxidation of ferrocytochrome c; (2) the pH equilibration kinetics of a respiration-induced pH gradient across the bilayer are a simple function of the ohmic proton-conductance properties of the membran...
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We describe effects of a mutation, Ile-67-->Asn, in subunit I of yeast cytochrome c oxidase on redox-linked protonation processes within the protein. The mutation lowers the midpoint potential of haem a and weakens its pH dependency, but has little effect on the potential of haem a3. The residue is close to a conserved glutamate (Glu-243) in the crystal structure. We propose that protonation of...
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Cytochrome c oxidase is an intricate metalloprotein that transfers electrons from cytochrome c to oxygen in the last step of the mitochondrial respiratory chain. It uses the free energy of this reaction to sustain a transmembrane electrochemical gradient of protons. Site-directed mutagenesis studies of bacterial terminal oxidases and the recent availability of refined crystal structures of the ...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Bioenergetics
سال: 2016
ISSN: 0005-2728
DOI: 10.1016/j.bbabio.2016.03.024